50.7 Folate:B12

50.7.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 131.619724 1 <0.0001 ***
folate_B12 2.967510 1 0.08 ns
Study 1.329267 1 0.2 ns
GA_samp 131.577880 1 <0.0001 ***
folate_B12:Study 5.013235 1 0.03 *
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 32.3040552 1 <0.0001 ***
folate_B12 0.1638235 1 0.7 ns
Study 9.3893442 1 0.002 **
GA_samp 1638.1363304 1 <0.0001 ***
folate_B12:Study 0.0023293 1 1.0 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 320.4006010 1 <0.0001 ***
folate_B12 1.3497714 1 0.2 ns
Study 0.1266652 1 0.7 ns
GA_samp 807.9421439 1 <0.0001 ***
folate_B12:Study 0.0677801 1 0.8 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.333 0.333 0.6 ns
3 Study 1 Inf 9.600 9.600 0.002 **
4 GA_samp 1 Inf 0.076 0.076 0.8 ns
2 folate_B12:Study 1 Inf 0.120 0.120 0.7 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.406 0.406 0.5 ns
3 Study 1 Inf 17.721 17.721 <0.0001 ***
4 GA_samp 1 Inf 0.153 0.153 0.7 ns
2 folate_B12:Study 1 Inf 0.217 0.217 0.6 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.412 0.412 0.5 ns
3 Study 1 Inf 44.737 44.737 <0.0001 ***
4 GA_samp 1 Inf 0.213 0.213 0.6 ns
2 folate_B12:Study 1 Inf 1.231 1.231 0.3 ns

50.7.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 13.46 (8.64, 20.98) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.09
StudySTOP 0.91 (0.77, 1.07) 0.25
GA_samp 1.17 (1.14, 1.20) <0.0001
folate_B12:StudySTOP 1.00 (1.00, 1.00) 0.03
hPL (μg/ml)
Var Effect P
(Intercept) 1.94 (1.55, 2.43) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.49
StudySTOP 1.14 (1.07, 1.23) 0.0002
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.08) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.13
StudySTOP 1.03 (0.94, 1.13) 0.52
GA_samp 1.32 (1.29, 1.34) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 15.64 (8.87, 27.58) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.60
StudySTOP 0.71 (0.59, 0.86) 0.0003
GA_samp 0.99 (0.96, 1.03) 0.79
HOMA-IR
Var Effect P
(Intercept) 3.91 (2.16, 7.07) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.58
StudySTOP 0.62 (0.51, 0.75) <0.0001
GA_samp 0.99 (0.96, 1.03) 0.70
Glucose (mmol/L)
Var Effect P
(Intercept) 5.53 (4.96, 6.16) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.57
StudySTOP 0.87 (0.84, 0.90) <0.0001
GA_samp 1.00 (0.99, 1.01) 0.61

50.7.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:B12 ratio and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 95.45434 7.888834 102.11034 81.02226 112.4571
STOP 97.32665 7.521490 78.50036 83.44885 113.5124
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 89.70666 2.645779 184.2667 84.63571 95.08143
STOP 102.63004 2.675395 112.7167 97.46401 108.06989
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.271708 0.0755750 406.0961 2.127895 2.425241
STOP 2.342068 0.0633228 205.9442 2.220493 2.470299
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.41229 0.9070753 Inf 12.739738 16.30443
STOP 10.28088 0.4603074 Inf 9.417142 11.22383
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.481303 0.2322185 Inf 3.05466 3.967535
STOP 2.146647 0.1004986 Inf 1.95844 2.352940
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.423639 0.0673084 Inf 5.293309 5.557179
STOP 4.717079 0.0405035 Inf 4.638358 4.797136

50.7.4 Comparisons

Effect for every 100 unit increase in Folate:B12 ratio.
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) folate_B12247.58 / folate_B12147.58 SCOPE 0.96 (0.93, 1.01) 0.09
PRL (ng/ml) folate_B12247.58 / folate_B12147.58 STOP 1.02 (0.99, 1.06) 0.2
hPL (μg/ml) folate_B12247.58 / folate_B12147.58 SCOPE 1.00 (0.98, 1.01) 0.5
hPL (μg/ml) folate_B12247.58 / folate_B12147.58 STOP 1.00 (0.98, 1.01) 0.5
GH2 (ng/ml) folate_B12247.58 / folate_B12147.58 SCOPE 0.99 (0.97, 1.00) 0.1
GH2 (ng/ml) folate_B12247.58 / folate_B12147.58 STOP 0.99 (0.97, 1.00) 0.1
Insulin (uU/ml) folate_B12247.58 / folate_B12147.58 SCOPE 0.99 (0.96, 1.02) 0.6
Insulin (uU/ml) folate_B12247.58 / folate_B12147.58 STOP 0.99 (0.96, 1.02) 0.6
HOMA-IR folate_B12247.58 / folate_B12147.58 SCOPE 0.99 (0.96, 1.02) 0.6
HOMA-IR folate_B12247.58 / folate_B12147.58 STOP 0.99 (0.96, 1.02) 0.6
Glucose (mmol/L) folate_B12247.58 / folate_B12147.58 SCOPE 1.00 (0.99, 1.01) 0.6
Glucose (mmol/L) folate_B12247.58 / folate_B12147.58 STOP 1.00 (0.99, 1.01) 0.6

50.7.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

50.7.6 Plots